diff --git a/packages/frontend/core/src/blocksuite/ai/components/ai-item/ai-item-list.ts b/packages/frontend/core/src/blocksuite/ai/components/ai-item/ai-item-list.ts
index af10ff413..ae4efe57a 100644
--- a/packages/frontend/core/src/blocksuite/ai/components/ai-item/ai-item-list.ts
+++ b/packages/frontend/core/src/blocksuite/ai/components/ai-item/ai-item-list.ts
@@ -1,5 +1,6 @@
 import { createLitPortal } from '@blocksuite/affine/components/portal';
 import { WithDisposable } from '@blocksuite/affine/global/lit';
+import { ColorScheme } from '@blocksuite/affine/model';
 import {
   EditorHost,
   PropTypes,
@@ -123,6 +124,7 @@ export class AIItemList extends WithDisposable(LitElement) {
           item => item.name,
           item =>
             html`<ai-item
+              .theme=${this.theme}
               .onClick=${this.onClick}
               .item=${item}
               .host=${this.host}
@@ -145,6 +147,9 @@ export class AIItemList extends WithDisposable(LitElement) {
 
   @property({ attribute: 'data-testid', reflect: true })
   accessor testId = 'ai-item-list';
+
+  @property({ attribute: false })
+  accessor theme: ColorScheme = ColorScheme.Light;
 }
 
 declare global {
diff --git a/packages/frontend/core/src/blocksuite/ai/components/ai-item/ai-item.ts b/packages/frontend/core/src/blocksuite/ai/components/ai-item/ai-item.ts
index f5ff594bb..65f7fb777 100644
--- a/packages/frontend/core/src/blocksuite/ai/components/ai-item/ai-item.ts
+++ b/packages/frontend/core/src/blocksuite/ai/components/ai-item/ai-item.ts
@@ -1,5 +1,6 @@
 import { ArrowRightIcon, EnterIcon } from '@blocksuite/affine/components/icons';
 import { WithDisposable } from '@blocksuite/affine/global/lit';
+import { ColorScheme } from '@blocksuite/affine/model';
 import {
   EditorHost,
   PropTypes,
@@ -27,6 +28,7 @@ export class AIItem extends WithDisposable(LitElement) {
 
     return html`<div
       data-testid=${testId}
+      data-app-theme=${this.theme}
       class="menu-item ${className}"
       @pointerdown=${(e: MouseEvent) => e.stopPropagation()}
       @click=${() => {
@@ -59,6 +61,9 @@ export class AIItem extends WithDisposable(LitElement) {
 
   @property({ attribute: false })
   accessor onClick: (() => void) | undefined;
+
+  @property({ attribute: false })
+  accessor theme: ColorScheme = ColorScheme.Light;
 }
 
 declare global {
diff --git a/packages/frontend/core/src/blocksuite/ai/components/ai-item/styles.ts b/packages/frontend/core/src/blocksuite/ai/components/ai-item/styles.ts
index 75236710e..4ffb5a416 100644
--- a/packages/frontend/core/src/blocksuite/ai/components/ai-item/styles.ts
+++ b/packages/frontend/core/src/blocksuite/ai/components/ai-item/styles.ts
@@ -1,4 +1,10 @@
-import { css } from 'lit';
+import { unsafeCSSVar, unsafeCSSVarV2 } from '@blocksuite/affine/shared/theme';
+import { darkCssVariables, lightCssVariables } from '@toeverything/theme';
+import {
+  darkCssVariablesV2,
+  lightCssVariablesV2,
+} from '@toeverything/theme/v2';
+import { css, unsafeCSS } from 'lit';
 
 export const menuItemStyles = css`
   .menu-item {
@@ -13,36 +19,41 @@ export const menuItemStyles = css`
     border-radius: 4px;
     box-sizing: border-box;
   }
+
   .menu-item:hover {
-    background: var(--affine-hover-color);
+    background: ${unsafeCSSVar('--affine-hover-color')};
     cursor: pointer;
   }
+
   .item-icon {
     display: flex;
-    color: var(--item-icon-color, var(--affine-brand-color));
+    color: ${unsafeCSSVar('--affine-brand-color')};
 
     svg {
       width: 20px;
       height: 20px;
     }
   }
+
   .menu-item:hover .item-icon {
-    color: var(--item-icon-hover-color, var(--affine-brand-color));
+    color: ${unsafeCSSVar('--affine-brand-color')};
   }
+
   .menu-item.discard:hover {
-    background: var(--affine-background-error-color);
+    background: ${unsafeCSSVar('--affine-background-error-color')};
     .item-name,
     .item-icon,
     .enter-icon {
-      color: var(--affine-error-color);
+      color: ${unsafeCSSVar('--affine-error-color')};
     }
   }
+
   .item-name {
     display: flex;
     padding: 0px 4px;
     align-items: baseline;
     flex: 1 0 0;
-    color: var(--affine-text-primary-color);
+    color: ${unsafeCSSVarV2('text/primary')};
     text-align: start;
     white-space: nowrap;
     font-feature-settings:
@@ -55,7 +66,7 @@ export const menuItemStyles = css`
   }
 
   .item-beta {
-    color: var(--affine-text-secondary-color);
+    color: ${unsafeCSSVarV2('text/secondary')};
     font-size: var(--affine-font-xs);
     font-weight: 500;
     margin-left: 0.5em;
@@ -63,14 +74,66 @@ export const menuItemStyles = css`
 
   .enter-icon,
   .arrow-right-icon {
-    color: var(--affine-icon-color);
+    color: ${unsafeCSSVarV2('icon/primary')};
     display: flex;
   }
+
   .enter-icon {
     opacity: 0;
   }
+
   .arrow-right-icon,
   .menu-item:hover .enter-icon {
     opacity: 1;
   }
+
+  .menu-item[data-app-theme='light'] {
+    .item-name {
+      color: ${unsafeCSS(lightCssVariablesV2['--affine-v2-text-primary'])};
+    }
+
+    .item-beta {
+      color: ${unsafeCSS(lightCssVariablesV2['--affine-v2-text-secondary'])};
+    }
+
+    .enter-icon,
+    .arrow-right-icon {
+      color: ${unsafeCSS(lightCssVariablesV2['--affine-v2-icon-primary'])};
+    }
+  }
+
+  .menu-item[data-app-theme='light']:hover {
+    background: ${unsafeCSS(lightCssVariables['--affine-hover-color'])};
+  }
+
+  .menu-item.discard[data-app-theme='light']:hover {
+    background: ${unsafeCSS(
+      lightCssVariables['--affine-background-error-color']
+    )};
+  }
+
+  .menu-item[data-app-theme='dark'] {
+    .item-name {
+      color: ${unsafeCSS(darkCssVariablesV2['--affine-v2-text-primary'])};
+    }
+
+    .item-beta {
+      color: ${unsafeCSS(darkCssVariablesV2['--affine-v2-text-secondary'])};
+    }
+
+    .enter-icon,
+    .arrow-right-icon {
+      color: ${unsafeCSS(darkCssVariablesV2['--affine-v2-icon-primary'])};
+    }
+  }
+
+  .menu-item[data-app-theme='dark']:hover {
+    background: ${unsafeCSS(darkCssVariables['--affine-hover-color'])};
+  }
+
+  .menu-item.discard[data-app-theme='dark']:hover {
+    background: ${unsafeCSS(
+      darkCssVariables['--affine-background-error-color']
+    )};
+  }
 `;
diff --git a/packages/frontend/core/src/blocksuite/ai/components/ask-ai-panel.ts b/packages/frontend/core/src/blocksuite/ai/components/ask-ai-panel.ts
index 2d9e4777d..20fa7abd6 100644
--- a/packages/frontend/core/src/blocksuite/ai/components/ask-ai-panel.ts
+++ b/packages/frontend/core/src/blocksuite/ai/components/ask-ai-panel.ts
@@ -1,5 +1,8 @@
 import { WithDisposable } from '@blocksuite/affine/global/lit';
-import { DocModeProvider } from '@blocksuite/affine/shared/services';
+import {
+  DocModeProvider,
+  ThemeProvider,
+} from '@blocksuite/affine/shared/services';
 import { scrollbarStyle } from '@blocksuite/affine/shared/styles';
 import { type EditorHost } from '@blocksuite/affine/std';
 import { cssVar } from '@toeverything/theme';
@@ -70,12 +73,23 @@ export class AskAIPanel extends WithDisposable(LitElement) {
     return filteredConfig;
   }
 
+  override connectedCallback() {
+    super.connectedCallback();
+    this.disposables.add(
+      this.host.std.get(ThemeProvider).app$.subscribe(() => {
+        this.requestUpdate();
+      })
+    );
+  }
+
   override render() {
     const style = styleMap({
       minWidth: `${this.minWidth}px`,
     });
+    const appTheme = this.host.std.get(ThemeProvider).app$.value;
     return html`<div class="ask-ai-panel" style=${style}>
       <ai-item-list
+        .theme=${appTheme}
         .host=${this.host}
         .groups=${this._actionGroups}
         .onClick=${this.onItemClick}
diff --git a/packages/frontend/core/src/blocksuite/ai/widgets/ai-panel/ai-panel.ts b/packages/frontend/core/src/blocksuite/ai/widgets/ai-panel/ai-panel.ts
index 70de65ae4..c8960772d 100644
--- a/packages/frontend/core/src/blocksuite/ai/widgets/ai-panel/ai-panel.ts
+++ b/packages/frontend/core/src/blocksuite/ai/widgets/ai-panel/ai-panel.ts
@@ -2,6 +2,7 @@ import {
   AFFINE_VIEWPORT_OVERLAY_WIDGET,
   type AffineViewportOverlayWidget,
 } from '@blocksuite/affine/blocks/root';
+import { ColorScheme } from '@blocksuite/affine/model';
 import {
   DocModeProvider,
   NotificationProvider,
@@ -9,6 +10,7 @@ import {
   ToolbarFlag,
   ToolbarRegistryIdentifier,
 } from '@blocksuite/affine/shared/services';
+import { unsafeCSSVar } from '@blocksuite/affine/shared/theme';
 import {
   getPageRootByElement,
   stopPropagation,
@@ -27,7 +29,8 @@ import {
   type Rect,
   shift,
 } from '@floating-ui/dom';
-import { css, html, nothing, type PropertyValues } from 'lit';
+import { darkCssVariables, lightCssVariables } from '@toeverything/theme';
+import { css, html, nothing, type PropertyValues, unsafeCSS } from 'lit';
 import { property, query } from 'lit/decorators.js';
 import { choose } from 'lit/directives/choose.js';
 import { literal, unsafeStatic } from 'lit/static-html.js';
@@ -43,9 +46,10 @@ export class AffineAIPanelWidget extends WidgetComponent {
       display: flex;
       outline: none;
       border-radius: var(--8, 8px);
-      border: 1px solid var(--affine-border-color);
-      background: var(--affine-background-overlay-panel-color);
-      box-shadow: var(--affine-overlay-shadow);
+      border: 1px solid;
+      border-color: ${unsafeCSSVar('--affine-border-color')};
+      background: ${unsafeCSSVar('backgroundOverlayPanelColor')};
+      box-shadow: ${unsafeCSSVar('overlayShadow')};
 
       position: absolute;
       width: max-content;
@@ -58,6 +62,21 @@ export class AffineAIPanelWidget extends WidgetComponent {
       --affine-font-family: var(--affine-font-sans-family);
     }
 
+    :host([data-app-theme='light']) {
+      background: ${unsafeCSS(
+        lightCssVariables['--affine-background-overlay-panel-color']
+      )};
+      border-color: ${unsafeCSS(lightCssVariables['--affine-border-color'])};
+      box-shadow: ${unsafeCSS(lightCssVariables['--affine-overlay-shadow'])};
+    }
+    :host([data-app-theme='dark']) {
+      background: ${unsafeCSS(
+        darkCssVariables['--affine-background-overlay-panel-color']
+      )};
+      border-color: ${unsafeCSS(darkCssVariables['--affine-border-color'])};
+      box-shadow: ${unsafeCSS(darkCssVariables['--affine-overlay-shadow'])};
+    }
+
     .ai-panel-container {
       display: flex;
       flex-direction: column;
@@ -414,6 +433,13 @@ export class AffineAIPanelWidget extends WidgetComponent {
 
   override connectedCallback() {
     super.connectedCallback();
+    this.appTheme = this.std.get(ThemeProvider).app$.value;
+    this.disposables.add(
+      this.std.get(ThemeProvider).app$.subscribe(theme => {
+        this.appTheme = theme;
+        this.requestUpdate();
+      })
+    );
 
     this.tabIndex = -1;
     // No need to synchronize the contents of the input into the editor.
@@ -460,7 +486,7 @@ export class AffineAIPanelWidget extends WidgetComponent {
     if (!this.config) return nothing;
     const config = this.config;
 
-    const theme = this.std.get(ThemeProvider).theme;
+    const theme = this.std.get(ThemeProvider).app$.value;
     const mainTemplate = choose(this.state, [
       [
         'input',
@@ -470,6 +496,7 @@ export class AffineAIPanelWidget extends WidgetComponent {
             .onFinish=${this._inputFinish}
             .onInput=${this.onInput}
             .networkSearchConfig=${config.networkSearchConfig}
+            .theme=${theme}
           ></ai-panel-input>`,
       ],
       [
@@ -564,6 +591,9 @@ export class AffineAIPanelWidget extends WidgetComponent {
 
   @property()
   accessor state: AffineAIPanelState = 'hidden';
+
+  @property({ attribute: 'data-app-theme', reflect: true })
+  accessor appTheme: ColorScheme = ColorScheme.Light;
 }
 
 export const aiPanelWidget = WidgetViewExtension(
diff --git a/packages/frontend/core/src/blocksuite/ai/widgets/ai-panel/components/state/input.ts b/packages/frontend/core/src/blocksuite/ai/widgets/ai-panel/components/state/input.ts
index cebc13178..89d07e221 100644
--- a/packages/frontend/core/src/blocksuite/ai/widgets/ai-panel/components/state/input.ts
+++ b/packages/frontend/core/src/blocksuite/ai/widgets/ai-panel/components/state/input.ts
@@ -1,9 +1,14 @@
 import { AIStarIcon } from '@blocksuite/affine/components/icons';
 import { SignalWatcher, WithDisposable } from '@blocksuite/affine/global/lit';
+import { ColorScheme } from '@blocksuite/affine/model';
 import { unsafeCSSVarV2 } from '@blocksuite/affine/shared/theme';
 import { stopPropagation } from '@blocksuite/affine/shared/utils';
 import { PublishIcon, SendIcon } from '@blocksuite/icons/lit';
-import { css, html, LitElement, nothing } from 'lit';
+import {
+  darkCssVariablesV2,
+  lightCssVariablesV2,
+} from '@toeverything/theme/v2';
+import { css, html, LitElement, nothing, unsafeCSS } from 'lit';
 import { property, query, state } from 'lit/decorators.js';
 
 import type { AINetworkSearchConfig } from '../../type';
@@ -20,7 +25,6 @@ export class AIPanelInput extends SignalWatcher(WithDisposable(LitElement)) {
       display: flex;
       align-items: flex-start;
       gap: 8px;
-      background: var(--affine-background-overlay-panel-color);
     }
 
     .star {
@@ -47,7 +51,7 @@ export class AIPanelInput extends SignalWatcher(WithDisposable(LitElement)) {
         overflow: hidden;
         padding: 0px;
 
-        color: var(--affine-text-primary-color);
+        color: ${unsafeCSSVarV2('text/primary')};
 
         /* light/sm */
         font-family: var(--affine-font-family);
@@ -58,11 +62,11 @@ export class AIPanelInput extends SignalWatcher(WithDisposable(LitElement)) {
       }
 
       textarea::placeholder {
-        color: var(--affine-placeholder-color);
+        color: ${unsafeCSSVarV2('text/placeholder')};
       }
 
       textarea::-moz-placeholder {
-        color: var(--affine-placeholder-color);
+        color: ${unsafeCSSVarV2('text/placeholder')};
       }
     }
 
@@ -79,12 +83,15 @@ export class AIPanelInput extends SignalWatcher(WithDisposable(LitElement)) {
         color: ${unsafeCSSVarV2('button/pureWhiteText')};
       }
     }
+
     .arrow[data-active] {
       background: ${unsafeCSSVarV2('icon/activated')};
     }
+
     .arrow[data-active]:hover {
       cursor: pointer;
     }
+
     .network {
       display: flex;
       align-items: center;
@@ -97,9 +104,90 @@ export class AIPanelInput extends SignalWatcher(WithDisposable(LitElement)) {
         color: ${unsafeCSSVarV2('icon/primary')};
       }
     }
+
     .network[data-active='true'] svg {
       color: ${unsafeCSSVarV2('icon/activated')};
     }
+
+    :host([data-app-theme='light']) {
+      .network svg {
+        color: ${unsafeCSS(lightCssVariablesV2['--affine-v2-icon-primary'])};
+      }
+
+      .network[data-active='true'] svg {
+        color: ${unsafeCSS(lightCssVariablesV2['--affine-v2-icon-activated'])};
+      }
+
+      textarea {
+        color: ${unsafeCSS(lightCssVariablesV2['--affine-v2-text-primary'])};
+      }
+
+      textarea::placeholder {
+        color: ${unsafeCSS(
+          lightCssVariablesV2['--affine-v2-text-placeholder']
+        )};
+      }
+
+      textarea::-moz-placeholder {
+        color: ${unsafeCSS(
+          lightCssVariablesV2['--affine-v2-text-placeholder']
+        )};
+      }
+
+      .arrow {
+        background: ${unsafeCSS(
+          lightCssVariablesV2['--affine-v2-icon-disable']
+        )};
+      }
+
+      .arrow[data-active] {
+        background: ${unsafeCSS(
+          lightCssVariablesV2['--affine-v2-icon-activated']
+        )};
+      }
+
+      .arrow svg {
+        color: ${unsafeCSS(lightCssVariablesV2['--affine-v2-pure-white-text'])};
+      }
+    }
+
+    :host([data-app-theme='dark']) {
+      .network svg {
+        color: ${unsafeCSS(darkCssVariablesV2['--affine-v2-icon-primary'])};
+      }
+
+      .network[data-active='true'] svg {
+        color: ${unsafeCSS(darkCssVariablesV2['--affine-v2-icon-activated'])};
+      }
+
+      textarea {
+        color: ${unsafeCSS(darkCssVariablesV2['--affine-v2-text-primary'])};
+      }
+
+      textarea::placeholder {
+        color: ${unsafeCSS(darkCssVariablesV2['--affine-v2-text-placeholder'])};
+      }
+
+      textarea::-moz-placeholder {
+        color: ${unsafeCSS(darkCssVariablesV2['--affine-v2-text-placeholder'])};
+      }
+
+      .arrow {
+        background: ${unsafeCSS(
+          darkCssVariablesV2['--affine-v2-icon-disable']
+        )};
+      }
+
+      .arrow[data-active] {
+        background: ${unsafeCSS(
+          darkCssVariablesV2['--affine-v2-icon-activated']
+        )};
+      }
+
+      .arrow svg {
+        color: ${unsafeCSS(darkCssVariablesV2['--affine-v2-pure-white-text'])};
+      }
+    }
   `;
 
   private readonly _onInput = () => {
@@ -209,6 +297,9 @@ export class AIPanelInput extends SignalWatcher(WithDisposable(LitElement)) {
   @property({ attribute: false })
   accessor onInput: ((input: string) => void) | undefined = undefined;
 
+  @property({ attribute: 'data-app-theme', reflect: true })
+  accessor theme: ColorScheme = ColorScheme.Light;
+
   @query('textarea')
   accessor textarea!: HTMLTextAreaElement;
 }
diff --git a/packages/frontend/core/src/blocksuite/ai/widgets/edgeless-copilot-panel/index.ts b/packages/frontend/core/src/blocksuite/ai/widgets/edgeless-copilot-panel/index.ts
index 90b448d72..8b1914c74 100644
--- a/packages/frontend/core/src/blocksuite/ai/widgets/edgeless-copilot-panel/index.ts
+++ b/packages/frontend/core/src/blocksuite/ai/widgets/edgeless-copilot-panel/index.ts
@@ -1,8 +1,11 @@
 import { WithDisposable } from '@blocksuite/affine/global/lit';
+import { ThemeProvider } from '@blocksuite/affine/shared/services';
 import { scrollbarStyle } from '@blocksuite/affine/shared/styles';
+import { unsafeCSSVar } from '@blocksuite/affine/shared/theme';
 import { on, stopPropagation } from '@blocksuite/affine/shared/utils';
 import type { EditorHost } from '@blocksuite/affine/std';
-import { css, html, LitElement, nothing } from 'lit';
+import { darkCssVariables, lightCssVariables } from '@toeverything/theme';
+import { css, html, LitElement, nothing, unsafeCSS } from 'lit';
 import { property } from 'lit/decorators.js';
 
 import type { AIItemGroupConfig } from '../../components/ai-item/types';
@@ -20,16 +23,44 @@ export class EdgelessCopilotPanel extends WithDisposable(LitElement) {
       min-width: 330px;
       max-height: 374px;
       overflow-y: auto;
-      background: var(--affine-background-overlay-panel-color);
-      box-shadow: var(--affine-shadow-2);
+      background: ${unsafeCSSVar('--affine-background-overlay-panel-color')};
+      box-shadow: ${unsafeCSSVar('--affine-overlay-shadow')};
       border-radius: 8px;
       z-index: var(--affine-z-index-popover);
     }
 
+    .edgeless-copilot-panel[data-app-theme='light'] {
+      background: ${unsafeCSS(
+        lightCssVariables['--affine-background-overlay-panel-color']
+      )};
+
+      box-shadow: ${unsafeCSS(lightCssVariables['--affine-overlay-shadow'])};
+    }
+
+    .edgeless-copilot-panel[data-app-theme='dark'] {
+      background: ${unsafeCSS(
+        darkCssVariables['--affine-background-overlay-panel-color']
+      )};
+
+      box-shadow: ${unsafeCSS(darkCssVariables['--affine-overlay-shadow'])};
+    }
+
+    .edgeless-copilot-panel[data-app-theme='dark'] ai-item {
+      background: blue;
+    }
+
     ${scrollbarStyle('.edgeless-copilot-panel')}
     .edgeless-copilot-panel:hover::-webkit-scrollbar-thumb {
       background-color: var(--affine-black-30);
     }
+
+    .edgeless-copilot-panel[data-app-theme='light']:hover::-webkit-scrollbar-thumb {
+      background-color: ${unsafeCSS(lightCssVariables['--affine-black30'])};
+    }
+
+    .edgeless-copilot-panel[data-app-theme='dark']:hover::-webkit-scrollbar-thumb {
+      background-color: ${unsafeCSS(darkCssVariables['--affine-black30'])};
+    }
   `;
 
   private _getChain() {
@@ -40,6 +71,11 @@ export class EdgelessCopilotPanel extends WithDisposable(LitElement) {
     super.connectedCallback();
     this._disposables.add(on(this, 'wheel', stopPropagation));
     this._disposables.add(on(this, 'pointerdown', stopPropagation));
+    this.disposables.add(
+      this.host.std.get(ThemeProvider).app$.subscribe(() => {
+        this.requestUpdate();
+      })
+    );
   }
 
   hide() {
@@ -47,6 +83,7 @@ export class EdgelessCopilotPanel extends WithDisposable(LitElement) {
   }
 
   override render() {
+    const appTheme = this.host.std.get(ThemeProvider).app$.value;
     const chain = this._getChain();
     const groups = this.groups.reduce((pre, group) => {
       const filtered = group.items.filter(item =>
@@ -61,8 +98,9 @@ export class EdgelessCopilotPanel extends WithDisposable(LitElement) {
     if (groups.every(group => group.items.length === 0)) return nothing;
 
     return html`
-      <div class="edgeless-copilot-panel">
+      <div class="edgeless-copilot-panel" data-app-theme=${appTheme}>
         <ai-item-list
+          .theme=${appTheme}
           .onClick=${() => {
             this.onClick?.();
           }}